COE

Ayurveda Formulations - Candraprabha vati, Tribhuvanakirti rasa, Karpuradi Curna, Navayasa Curna, Vaisvanara Curna, Sarasvatha Curna, Kalyana Ksara, Anandabhairava Rasa, Brhat Vatachinthamani rasa, Svasananda Gutika, Vatavidhvamsana rasa, Smritisagara rasa, Kasturi matralu, Lasunadi vati and Kantavallabha rasa

Siddha Formulations - Ayakaantha Centuram, Padigalinga Centuram, Chanda Maarutha Centuram, Padigara Parpam and Sangu Parpam

Ayurveda Formulations - Arogya Vardhini Gutika, Abhraka Bhasma, Prabhakara Vati, Dhatri Lauha, Punarnavadi Mandura, Suvarnamakshika bhasma, Kassesa Bhasma, Rajatha bhasma, Amavatari rasa, Vatagajangusa rasa, Chandramirtha rasa, Ekangivira rasa, Laghu Malivasanta Rasa, Hridayarnav rasa and Sringarabha rasa

Siddha Formulations - Gowri Chinthamani Chenduram, Thalaga karuppu, Naga parpam, Brahmananda bhairavam and Karuppu Vishnuchakra mathirai

Ayurveda Formulations - Arogavardhini Gutika, Abhraka Bhasma, Prabhakara Vati, Dhatri Lauha, Punarnavadi Mandura, Svarnamaksika Bhasma, Kasisa Bhasma, Rajata Bhasma, Candramrta Rasa, Ekangavira Rasa, Laghu Malinivasanta Rasa, Hrdayarnava Rasa, Srngarabha Rasa, Amavatari Rasa, Vatagajankusa Rasa

Siddha Formulations - Gowri Chinthamani Chenduram, ThalagaKaruppu, Naka parpam, Brahmananda bhairavam, Karuppu Vishnuchakra mathirai

• Affymetrix Microarray System (M/s. Imperial Life Sciences (P) Ltd)
• Zeta sizer (Nano-ZS, Malvern Instruments, UK)
• Phase contrast microscope with fluorescence (AxioScope. A1, Carl Zeiss, Germany)
• Laminar flow chamber (NU-437-400S Labgard, Nuaire, USA)
• Rotary flash vacuum evaporator (Alpha 2-4 LD, Martin Christ, Germany)
• 2D electrophoresis (IEF100, Hoefer, USA)
• Double beam UV-Vis spectrophotometer (ND-2000, Thermoelectron Scientific Instruments, USA)
• QSAR Software (Schrodinger, Germany)
• Water Purification System (M/s. Evoqua Water Technologies, Singapore)
• Refrigerated centrifuge (5810 R, Eppendorf, Germany)
• Voltage stabilizers

• Completed Physico-chemical characterization of 20 formulations (15 Ayurveda and 5 Siddha) that revealed the inorganic constituents in the samples.
• In vivo studies to evaluate the safety of Candraprabha Vati and Tribhuvanakirti Rasa preparations in male and female Wistar rats administered with repeated doses for 28 days was carried out which revealed no significant adverse effects of both formulations in the therapeutic doses.
• Microarray analysis of animals administered with Candraprabha Vati has been performed for the first time
• Microarray data analysis has revealed several molecular targets that might be responsible for the therapeutic effects of CPV
  • miR-434: may play a role in the anti-diabetic and anti-inflammatory activities of CPV
  • MeOX1 and Upf3b: may contribute to the rejuvenative properties of CPV
  • Osbpl7: may be involved in the therapeutic effect of CPV in hepatic disorders
  • miR877: may have a role in CPV’s therapeutic effect against fibrosis and associated complications
  • Hbaa2: may contribute to the anti-anemic effect of CPV
  • miRlet7e: may play a role in the anti-inflammatory property of CPV
• Microarray data also revealed a gender-specific regulation of the genes with the female animals showing a marked alteration in Rab3d gene that may have a role in anti-carcinogenic property of CPV when compared male animals
• 6 research publications emerged out of the COE project – 4 publications under 1st installment and 2 publications under 2nd installment

Training Programmes

• Six days training programme on "Characterization techniques in the Standardization of Herbo-Metallic Preparations" was organized from 27th April to 02nd May 2015
• Three days training programme on "Characterization techniques in the Standardization of Herbo-Metallic Preparations" was organized from 28th to 30th March 2016

Seminars/Conferences organized

• An International Conference, "Phytocongress" was organized on March 7 & 8, 2013 with participation of over 200 delegates
• An International Conference, Phytocongress 2016 - "Indian Traditional Medicine - A Conglomeration of Ancient Knowledge and Modern Science" was organized on July 21 & 22, 2016 with participation of over 200 delegates

Milestones with deliverables achieved during the reporting period as proposed in the scheme (COE – 3^rd installment)

Physico-Chemical Characterization of Rasa Aushadhies and Bhasmas

• Completed the study on physico-chemical characterization of 15 Ayurveda formulations (Arogya Vardhini Gutika, Abhraka Bhasma, Prabhakara Vati, Dhatri Lauha, Punarnavadi Mandura, Suvarnamakshika bhasma, Kassesa Bhasma, Rajatha bhasma, Amavatari rasa, Vatagajangusa rasa, Chandramirtha rasa, Ekangivira rasa, Laghu Malivasanta Rasa, Hridayarnav rasa and Sringarabha rasa)
• Completed five Siddha formulations (Gowri Chinthamani Chenduram, Thalaga karuppu, Naga parpam, Brahmananda bhairavam and Karuppu Vishnuchakra mathirai).

Major Conclusions

• Physico-chemical characterization of 20 traditional formulations carried out revealed their morphology, elemental composition, crystallinity and chemical nature of interactions.
• Acute and Sub-acute toxicity studies carried out on 2 Herbo-metallic formulations, Arogya Vardhini Gutika and Punarnavadi mandura using rodent models revealed that both formulations were well tolerated with no adverse behavioral or organ level changes.
• The therapeutic efficacy of Punarnavadi mandura in hemolytic anemia model confirmed its efficacy through a combination of anti-inflammatory, activation of RBC production and inhibition of Ganma Gandy bodies.

Publications:

• Preparation and Characterization of Kowsigar Kuzhambhu, a Siddha Formulation. International Journal of Pharmacy and Pharmaceutical Sciences, Subash Chandra Bose A, Brindha Pemiah, Sridharan Krishnaswamy, Uma Maheswari Krishnan, Swaminathan Sethuraman, Rajan K Sekar, 2012, Vol 4 (2), pp:65-68
• Unraveling ancient medicinal formulation secrets: preparation of gandhagaparpam. International Journal of Pharmacy and Pharmaceutical Sciences, Grace Subha Nesapriya J, Rajalakshmi Perumal, Brindha Pemiah, Sridharan Krishnaswamy, Uma Maheswari Krishnan, Swaminathan Sethuraman, Rajan K. Sekar, 2012, Vol 4(2) pp: 60-64
• Preparation and Characterization of Chandraprabha Vati, An Ayurvedic Formulation. International Journal of Pharmacy and Pharmaceutical Sciences, Safiullah A, Devanathan Rengarajan, Brindha Pemiah, Sridharan Krishnaswamy, Uma Maheswari Krishnan, Swaminathan Sethuraman, Rajan K. Sekar, 2012, Vol. 4(2) pp 55-59
• Preparation & Characterization of Vanga Bhasma, A Tin-Based Herbo-Metallic Preparation. International Journal of Pharmacy and Pharmaceutical Sciences, Sushil Kumar Vishwakarma, Rajalakshmi Perumal, Brindha Pemaih, Sridharan Krishnaswamy, Uma Maheswari Krishnan, Swaminathan Sethuraman, Rajan K.Sekar, 2012, Vol.4(2) pp.49-54
• Transcriptional regulation of the pregnane-X receptor by the Ayurvedic formulation Chandraprabha Vati, RSC Advances, Stalin Selvaraj, Ramya Ramanathan, Varshini Vasudevaraja, Kalpoondi Sekar Rajan, Sridharan Krishnaswamy, Brindha Pemiah, Swaminathan Sethuraman, Vigneshwar Ramakrishnan and Uma Maheswari Krishnan, 2014, Issue 110, pp: 64967-64976 (IF: 3.119)
• Microarray analysis of genes from animals treated with a traditional formulation Chandraprabha vati reveals its therapeutic targets, Journal of Traditional and Complementary Medicine, January 2020; Pg 36-44. (IF: 3.5)
• Sriram, S., Vadivel, V., Rajalakshmi, P., Brindha, P. Standardization and Surface Characterization Studies of a Siddha Formulation: Chandamarutha centhuram, Asian Journal of Chemistry, May 2023.
• Rajendran, Kayalvizhi et al. “Therapeutic efficacy of Punarnavadi mandura against phenylhydrazine-induced hemolytic anemia in rats.” Journal of Traditional and Complementary Medicine (2024): (IF: 3.5)
• Sriram S., Savariraj Sahayam C., Rajalakshmi P., Brindha P. “Scientific Exploration of Nanoparticles in Gowri Chinthamani Chenthuram and its Quality Control Studies” Journal of Chemical Health Risks (2024)
• Rajalakshmi,P. Ravichandran, N. Sudha, V. Lavanya, R. Sriram, S. Brindha, P. “Physicochemical Profiling of Lasunadi Vati – A Polyherbal Ayurvedic Formulation” Journal of Chemical Health Risks (2024)

Training Programmes:

• An AYUSH Training Programme for two days on “Standardization and Scientific Validation techniques in understanding the Safety and Toxicity of Herbo-Metallic Preparations” was organized on 25.02.2022 and 26.02.2022
• An AYUSH Training Programme for two days on “Standardization and Scientific Validation techniques in understanding the Safety and Toxicity of Herbo-Metallic Preparations” was organized on 11.03.2022 and 12.03.2022.

• Preparation and Characterization of Kowsigar Kuzhambhu, a Siddha Formulation. International Journal of Pharmacy and Pharmaceutical Sciences, Subash Chandra Bose A, Brindha Pemiah, Sridharan Krishnaswamy, Uma Maheswari Krishnan, Swaminathan Sethuraman, Rajan K Sekar, 2012, Vol 4 (2), pp:65-68
• Unraveling ancient medicinal formulation secrets: preparation of gandhagaparpam. International Journal of Pharmacy and Pharmaceutical Sciences, Grace Subha Nesapriya J, Rajalakshmi Perumal, Brindha Pemiah, Sridharan Krishnaswamy, Uma Maheswari Krishnan, Swaminathan Sethuraman, Rajan K. Sekar, 2012, Vol 4(2) pp: 60-64
• Preparation and Characterization of Chandraprabha Vati, An Ayurvedic Formulation. International Journal of Pharmacy and Pharmaceutical Sciences, Safiullah A, Devanathan Rengarajan, Brindha Pemiah, Sridharan Krishnaswamy, Uma Maheswari Krishnan, Swaminathan Sethuraman, Rajan K. Sekar, 2012, Vol. 4(2) pp 55-59
• Preparation & Characterization of Vanga Bhasma, A Tin-Based Herbo-Metallic Preparation. International Journal of Pharmacy and Pharmaceutical Sciences, Sushil Kumar Vishwakarma, Rajalakshmi Perumal, Brindha Pemaih, Sridharan Krishnaswamy, Uma Maheswari Krishnan, Swaminathan Sethuraman, Rajan K.Sekar, 2012, Vol.4(2) pp.49-54
• Transcriptional regulation of the pregnane-X receptor by the Ayurvedic formulation Chandraprabha Vati, RSC Advances, Stalin Selvaraj, Ramya Ramanathan, Varshini Vasudevaraja, Kalpoondi Sekar Rajan, Sridharan Krishnaswamy, Brindha Pemiah, Swaminathan Sethuraman, Vigneshwar Ramakrishnan and Uma Maheswari Krishnan, 2014, Issue 110, pp: 64967-64976 (IF: 3.119)
• Microarray analysis of genes from animals treated with a traditional formulation Chandraprabha vati reveals its therapeutic targets, Journal of Traditional and Complementary Medicine, January 2020; Pg 36-44. (IF: 3.5)
• Sriram, S., Vadivel, V., Rajalakshmi, P., Brindha, P. Standardization and Surface Characterization Studies of a Siddha Formulation: Chandamarutha centhuram, Asian Journal of Chemistry, May 2023.
• Rajendran, Kayalvizhi et al. “Therapeutic efficacy of Punarnavadi mandura against phenylhydrazine-induced hemolytic anemia in rats.” Journal of Traditional and Complementary Medicine (2024): (IF: 3.5)
• Sriram S., Savariraj Sahayam C., Rajalakshmi P., Brindha P. “Scientific Exploration of Nanoparticles in Gowri Chinthamani Chenthuram and its Quality Control Studies” Journal of Chemical Health Risks (2024)
• Rajalakshmi,P. Ravichandran, N. Sudha, V. Lavanya, R. Sriram, S. Brindha, P. “Physicochemical Profiling of Lasunadi Vati – A Polyherbal Ayurvedic Formulation” Journal of Chemical Health Risks (2024)

Training programme 2015

The Centre for Advanced Research in Indian System of Medicine (CARISM), SASTRA University, organized a training program for six days on "Characterization techniques in the Standardization of Herbo-metallic preparations" under the Centre of Excellence Scheme sponsored by the Department of AYUSH, Government of India, New Delhi from 27.04.2015 to 02.05.2015. The training program was inaugurated by Dr. K. Uma Maheswari, Associate Dean, SCBT, SASTRA. The details of the lecture and practical sessions of the training program are given below:

20 participants from various Ayurveda and Siddha Institutes in Chennai, Puducherry, Palayamkottai and Erode attended the training program. The participants in their feedback mentioned that the six days training program was a great learning experience and also requested this program to be conducted every year.

Training programme 2016

The Centre for Advanced Research in Indian System of Medicine (CARISM), SASTRA University, organized a Training program for three days on “Characterization Techniques in the Standardization of Ayurveda and Siddha Herbo-Metallic Preparations” under the Centre of Excellence Scheme sponsored by the Department of AYUSH, Government of India, New Delhi from 28.03.2016 to 30.03.2016. The training program was inaugurated by Dr. K. Ravi, Joint Adviser, (Siddha), Ministry of Ayush, New Delhi. The details of the lecture and practical sessions of the training program are given below:

53 participants from various Ayurveda and Siddha Institutes in Chennai, Cuddalore, Madurai, Coimbatore, Kanchipuram, Palayamkottai and Bangalore attended the training program. The participants said that the program was very beneficial to them as it offered valuable theoretical and technical inputs on the role of modern characterization techniques in the standardization of herbo-metallic preparations.

List of Participants from AYUSH fraternity in Training Programme 2016

International Conference – 2016
The Centre for Advanced Research in Indian System of Medicine (CARISM), SASTRA University, organized an International Conference Phytocongress 2016 - “Indian Traditional Medicine - A Conglomeration of Ancient Knowledge and Modern Science” on 21 st and 22 nd July 2016. Dr. Bhushan Patwardhan, Professor and Director of Interdisciplinary School of Health Sciences, Savitribai Phule Pune University inaugurated the conference and said “Though interactions have been taking place between modern and Indian systems of medicine, the originality is mostly forgotten and we should harness the best from each of the system for the welfare of mankind. The details of the program schedule and plenary lecture sessions of the conference are given below:
21 July, 2016

22 July, 2016

About 194 participants that included academicians, students and research scholars from various eminent educational institutions across Tamil Nadu attended the International Conference. The participants thanked the management of SASTRA University and CARISM for organizing this conference as it provided them a golden opportunity to listen to and interact with several national and international academic giants.
Participants from AYUSH fraternity in Phytocongress 2016

Training Programmes 2022

1.Two-day Training programme on “Standardization and Scientific Validation techniques in understanding the Safety and Toxicity of Herbo-Metallic Preparations”

A 2-day training programme on “Standardization and Scientific Validation techniques in understanding the Safety and Toxicity of Herbo-Metallic Preparations” was conducted on 25.02.2022 and 26.02.2022. The schedule of the training programme was planned in such a way that the necessary conceptual details and background information were provided in the forenoon sessions, followed by practical demonstration sessions in the afternoon. The detailed programme is given below

Schedule of training programme on “Standardization and Scientific Validation techniques in understanding the Safety and Toxicity of Herbo-Metallic Preparations”

Keynote speakers from AYUSH fraternity

33 participants from various organizations such as Central Council for Research in Siddha(CCRS), Chennai, Siddha Central Research Institute (SCRI), Chennai, Ayurveda students from Nandha Ayurveda Medical College and Hospital, Erode and Research scholars from SASTRA Deemed to be University benefitted from this programme. The list of participants is given below

List of participants

2. Two-day Training programme on “Standardization and Scientific Validation techniques in understanding the Safety and Toxicity of Herbo-Metallic Preparations”

A 2-day training programme on “Standardization and Scientific Validation techniques in understanding the Safety and Toxicity of Herbo-Metallic Preparations” was conducted on 11.03.2022 and 12.03.2022. The detailed programme is given below

Schedule of training programme on “Standardization and Scientific Validation techniques in understanding the Safety and Toxicity of Herbo-Metallic Preparations”

Keynote speakers from AYUSH fraternity

24 participants from various organizations like Central Council for Research in Siddha (CCRS), Chennai, Siddha Central Research Institute (SCRI), Chennai, Siddha practitioners from the National Institute of Siddha, Chennai, Sri Sairam Siddha Medical College, Chennai and Tamil University, Thanjavur benefitted from this programme. The list of participants is given below

List of participants